A Systematic Study of Traditional Chinese Medicine for the Treatment of Lung Adenocarcinoma Using a Reverse Network of Key Targets Based on Bioinformatics and Molecular Docking: Curcumin and Trans-Resveratrol as Potential Drug Candidates for Lung Adenocarcinoma

Background Lung adenocarcinoma (LUAD) is a dominant tumor with high morbidity and mortality. In spite of innovations in surgery, chemotherapy, targeted therapies, the prognosis for patients LUAD remains unsatisfactory. Therefore, it particularly important to study biological markers occurrence, development, this disease. Methods study, 3 datasets were selected from Gene Expression Omnibus (GEO) database screen differentially expressed genes (DEGs). Then, Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) analyses performed using Database Annotation, Visualization Integrated Discovery (DAVID). addition, protein-protein interaction network was 10 key analyzed. protein expression analyzed The Cancer Genome Atlas (TCGA) Human Protein (HPA) databases, respectively. overall survival (OS) rate Kaplan-Meier plotter database. chemical components downloaded HERB Finally, constituents docked targets by molecular docking. By vitro studies, we evaluated effects curcumin trans-resveratrol on proliferation cells. Results A total 122 upregulated 434 downregulated identified. (glyceraldehyde-3-phosphate dehydrogenase (GAPDH), interleukin-1 beta (IL1B), von Willebrand factor (VWF), matrix metalloproteinase-9 (MMP9), CD44 antigen (CD44), transcription Jun (JUN), platelet endothelial cell adhesion molecule (PECAM1), hematopoietic progenitor CD34 (CD34), peroxisome proliferator-activated receptor gamma (PPARG), collagen alpha-1(I) chain (COL1A1)) obtained. TCGA results showed that GAPDH, MMP9, COL1A1 upregulated, while IL-1B, VWF, CD44, JUN, PECAM1, CD34, PPARG cancer tissues. analysis also PPARG, COL1A1, low associated poor LUAD. Combined gene expression, staging, immunohistochemistry, OS prognostic analysis, potential target occurrence 2 (curcumin trans-resveratrol) had good docking effect COL1A1. We found significantly inhibited cells decreased MMP9. Conclusion conclusion, our identified main traditional Chinese medicine treatment. This provides therapeutic treatment strategies